WP3: Enumeration methods for large molecular structures: application to lipoprotein testing

The aim of this workpackage is to develop advanced lipoprotein analysis methods to address the need for new comprehensive standards and reference systems capable of finely depicting the lipoprotein profile and provide new insights into the assessment of the CVD risk. Most recent advances in lipid testing indicate that the number of atherogenic particles and especially LDL particles (LDL-P) is linked more strongly to cardiovascular disease than the amount of cholesterol bound to them (LDL-C) and is a valuable adjunct target of therapy: knowing patient’s LDL-P, along with their LDL-C, gives a more complete picture to help the cardiovascular disease management.

This work package will develop novel metrological tools to address the need for new comprehensive standards and reference systems capable of finely depicting the lipid profile. A new candidate reference method will be developed for LDL-C measurement, based on implementation of Electrospray-Differential Mobility Analysis as primary method for the enumeration of the main atherogenic particle subclasses, and a candidate reference material will be produced. Interlaboratory studies with clinical material amongst stakeholders will be organised with input from interested collaborators.